The fungus Aspergillus niger is used to produce from glucose a specific enzyme, which is excreted in the medium. The glucose is used by the fungus for growth, maintenance and production (which is not growth-related).
The enzyme is produced in a fed-batch fermentation in a well-stirred tank of 50 m3 working volume. The fungus is first grown batch-wise in 20 m3. The inoculum is 5 kg and when 800 kg of fungus is reached in this batch-fermentation, the fed-batch is started and lasts for a period of 25 h.
Calculate how much glucose is consumed for product formation during the batch period.
